Human HMGB-1(High Mobility Group Protein B1) CLIA Kit (E-CL-H0997)

Name: Human HMGB-1(High Mobility Group Protein B1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0997
Price: 180.0 USD
Availability: InStock

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Human HMGB-1(High Mobility Group Protein B1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human HMGB-1 in samples.No significant cross-reactivity or interference between Human HMGB-1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	50084.000	58458.000	54271.000	54247
400	22068.000	22084.000	22076.000	22052
200	10854.000	10110.000	10482.000	10458
100	4686.000	5114.000	4900.000	4876
50	2451.000	2173.000	2312.000	2288
25	1244.000	1078.000	1161.000	1137
12.5	519.000	525.000	522.000	498
0	23.000	25.000	24.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	38.940	71.200	397.790	37.000	72.830	379.260
Standard deviation	4.740	8.160	27.920	3.480	5.480	28.710
CV（%）	12.170	11.460	7.020	9.410	7.520	7.570

Recovery

The recovery of Human HMGB-1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	99-111	104
EDTA plasma （n=8）	94-111	102
Cell culture media （n=8）	86-99	91

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human HMGB-1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	103-120	103-118	93-109
1:2	Average	109	109	101
1:4	Range	85-98	94-107	95-111
1:4	Average	90	99	103
1:8	Range	101-114	86-100	93-109
1:8	Average	108	93	99

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HMGB-1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human HMGB-1. You can calculate the concentration of Human HMGB-1 in the samples by comparing the RLU value of the samples to the standard curve.

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